What have we learned from scid mouse free#

Gene namesī2m is required for normal expression of major histocompatibility class I proteins (displaying viral and self antigens to potentially responsive T cells) and for CD8+ T cell maturation and NK cell development Gene names and functions for models in Table 1. NOD.Cg- Prkdc scid Il2rg tm1Wjl/SzJ ( 005557) NOD.Cg- Prkdc scid B2m tm1Unc/J ( 002570) Selected Immunodeficient JAX® Mice Models (stock number) Major features of selected backgrounds for JAX® Mice immunodeficient models and the names of selected models on those backgrounds Consider the kind of research you are conducting (allograft, xenograft, immunology, cancer, etc.) and how your research will relate to previous and future research. Nude, Rag1 null and Pkrdc scid mice should be housed in specific pathogen-free (SPF) environments. If it is not, consider requesting a Dedicated Supply contract with JAX® Services. Consider whether the mutant you want is readily available in the quantities you need. For example, the beta 2 microglobulin and perforin mutations lower NK cell activity, the interleukin 2 receptor, gamma chain mutation completely eliminates it, and the scid mutation renders NOD mice resistant to diabetes (Tables 2 and 3). Consider how the mutant gene affects immune responses (such as NK cell, macrophage and complement activity) and interacts with the genetic background of a mutant. Consider how the gene of interest functions, and where it is expressed (Table 2). Female nude mice are poor breeders: they begin to ovulate late, when 2.5 months old, and stop early, when 4 months old. Prkdc scid mice are sensitive to radiation and therefore cannot be as thoroughly irradiated as other immunodeficient models before being engrafted.

This limits their use for long-term experiments. Some immunodeficient mice die young because they are susceptible to thymic lymphomas (Table 3).

What have we learned from scid mouse free#

It is higher in mice housed under non-specific pathogen free (SPF) conditions, and it is generally high on the C57BL/6J and BALB/cByJ backgrounds, low on the C3H/HeSnJSmn background, and very low on the NOD/LtSzJ background (Table 1). As applied to Prkdc scid mice, leakiness refers to their tendency (on certain backgrounds) to produce some functional B and T cells as they age. Consider the activity of the mutant's endogenous B cells, T cells, NK cells, APCs and complement (Table 1). Functionality of various endogenous immune system components.For example, the NOD strain is susceptible to diabetes and is deficient in natural killer (NK), macrophage, antigen presenting cell (APC) and complement activity (Table 1). Consider important features of the background strain, such as H2 haplotype, behavior and disease susceptibility. Some of the major ones are listed below (Tables 1, 2, and 3 summarize how these factors apply to selected JAX® Mice models): To choose the most appropriate immunodeficient mouse model for your research, you will have to consider many factors. The purpose of this article is to help you make those choices. As the number, diversity and specialized research applications of these models increase, so does the difficulty of choosing the most appropriate ones for a particular study. Immunodeficient mouse models, particularly severe combined immunodeficient ( Prkdc scid and Rag1 null) mice, are very useful models for immunology, infectious disease, cancer, stem cell biology and other research.